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KMID : 0974620020200020477
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Bulletin of Dongnam Health University
2002 Volume.20 No. 2 p.477 ~ p.482
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Determination of PML/RARa Gene Rearrangement In Acute Promyelocytic Leukemia with Reverse Transcription Polymerase Chain Reaction
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Jo Yoon-Kyung
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Abstract
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The Molecular characterization of the translocations involved in hematopoietic tumors has advanced our understanding of neoplastic transformation. Acute promyelocytic leukemia(APL:AML M3) is identified by a unique t(15;17) translocation which fuses the PML gene to the retinoic acid receptor alpha gene(RARα) and a consistent translocation is present in the blasts of the majority of APL patients. The t(15;17) translocation generates a PML/RARα chimeric gene that is transcribed as a fusion PML/RARα messenger RNA. The detection of the PML/RARα rearrangements involved in APL has not only provided pathogenitic marker but also permitted more accurate diagnosis and monitoring of the patient’s response to therapy. I had examined the PML/RARα rearrangement by reverse transcriptase polymerase chain reaction (RT-PCR). In APL patients, I had detected long form type(second PCR product 370 bp) and short form type(first PCT product 221 bp) gene rearrangement.
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